pluriBead® Technology & Principle
pluriBead® uses non-magnetic monodispersed microparticles (beads) for the sorting of cell mixtures. The beads are larger than the cells and thus cannot be phagocytized by them. Their surface is modified with monoclonal antibodies that recognize specific structures on the target cell surface. During incubation, the target cells in suspension will bind to the pluriBeads® and can be separated afterwards by a pluriStrainer® (size exclusion) from the suspension. Pretreatment of the blood, such as the production of a mononuclear cell fraction (PBMC) or others (e.g. erythrolysis or target concentration), is not required.
No centrifugation: less mechanical stress, high cell viability
No magnets: combine antigen-antibody interaction with filtration techniques
Fast isolation: fast and easy to isolate cells within 10-30 minutes
Ready-to-use: kits contain all materials needed for cell separation
High performance: high cell purity (>98%) and high cell viability (>98%)
Multi function: simultaneously isolate two cell populations